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The tubes were capped tightly and incubated on a water bath at 85°C for 15 min. After incubation test tubes were uncapped and 1 ml of ice-cold TCA (17.5% w/v) was added immediately.
An additional incubation test on normal NH4 +-free agar was performed.
After incubation, test animals were washed again in PBS buffer and then placed on a glass slide.
The SBF incubation test confirmed that the fast formation of apatite-like materials suggests in vitro bioactive behavior of the nanofibers.
The SBF incubation test confirmed the faster deposition of large amount of calcium phosphate around the composite polyamide-6/calcium lactate fibers compared to pristine polyamide-6.
The SBF incubation test confirmed the formation of apatite-like materials, exhibiting enhanced bioactive behavior of the polyurethane/CaCl2 composite nanofibers.
Similar(47)
After 10 min of incubation, tests were initiated by adding ADP (10 μM) and AA (0.5 mM).
Only Coprinellus micaceus differed from the other species in that spore yields in both directions were comparable both after 18 h incubation and after shorter 8 h incubation tested also for this fungus (Table 1).
To avoid solubility problems in the incubation tests, PAHs were first dissolved in ethanol before digestion.
The stability of the MCs in the in vitro incubation tests could be due to their chemical structures.
No bactericidal activity (i.e., ≥3 log10 CFU/mL decrease) was observed at 1x MIC for any strain and time after incubation tested.
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