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After humidity treatment, the surface morphology and potential were rough and varied (around 198.26 mV variation), respectively (Figure 2d).

Before humidity treatment, the surface morphology and potential were smooth and almost constant (around 10 to 25 mV variation) by SEM and KPFM analysis, respectively (Figure 2c).

In the post-drying humidity treatment, a significant segregation between taurine and glycine was in a clear contrast to the uniform distribution in those without treatment.

After 72 hours of humidity treatment, the enclosed soil crusts reached equilibrium with respect to the water potential of the surrounding air, i.e. their weight (water content) no longer increased.

Finally, the thin film was turned into the organic inorganic hybrid material by humidity treatment of 90% RH at 50°C for 180 min. This temperature and humidity constituted the best condition for the sol gel reaction of PHPS recommended by a chemical company, Sanwa Kagaku Corp. (Nagoya, Japan).

The c-ZnO NWs surface passivation can slow down the interaction between the moisture solution and c-ZnO NWs surface; the passive c-ZnO NWs would not have the spontaneous reaction in the same humidity treatment, as seen in Figure 4a,b,c,d).

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There has been some study of flexural properties of hedgehog spines, but an understanding of how this keratinous biological material is affected by various temperature and relative humidity treatments, or how spine color (multicolored vs. white) affects mechanics, is lacking.

Each domicile treatment was replicated at 2 humidity treatments: high and low, by placing the tubes into incubators (Brinsea® Octagonal 40), one maintained at 70 80% r.h.

A few larvae drowned in condensation droplets in the high humidity treatments; data for these were censored at the point of drowning.

To the best of our knowledge, this is the first work that captured in real time the effects of post-humidity treatment on the morphological differentiation in spray-dried mixed amino acid particles.

To create a high-humidity treatment, glass containers similar to those used for flooding were lined with wet filter paper (standard filter paper; Schleicher and Schuell GmbH, Dassel, Germany).

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