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Slides were then washed as described above and incubated with a combination of goat anti-rabbit IgG conjugated to AF555 (Molecular probes, Carlsbad, CA, A21424, 1∶200) in goat anti-mouse Envision+ (DAKO, Carpinteria, CA) for 60 minutes at room temperature in a dark humidity tray.
Water in the humidity tray should be replaced weekly and the tray cleaned as above.
Adding disinfectants or chemicals to the water in the incubator humidity tray is not recommended as many of these will produce volatile compounds, which may be cytotoxic to the cultured cells.
Slides were incubated at 4°C overnight in a humidity tray with primary antibodies, VEGF-A (mouse monoclonal antibody, 1 100, DAKO), VEGF-C (goat polyclonal antibody, 1 100, Santa Cruz Biotechnology, Santa Cruz, CA), VEGF-D (goat polyclonal antibody, 1 100, Santa Cruz), VEGFR-3 (goat polyclonal antibody, 1 100, Santa Cruz) and D2-40 (monoclonalclonantibodyody, 1 100, DAKO), respectively.
Make your own humidity tray.
Set the humidity tray below the umbrella plant.
Similar(47)
The inoculated plants were kept 3 days under high humidity (covered trays) in the growth chamber.
The inoculated plants were kept under high humidity in covered trays.
SMS treatment leaves were incubated in covered trays at high humidity (referred to as humid conditions); in some cases the trays were left uncovered after SMS treatment (referred to as dry conditions) at the same laboratory conditions.
Because of approximately 1 2 weeks of uncertainty regarding the date when eggs were laid, staging was done according to the embryological series and stages described in [30] Chicken fertilized eggs were obtained from Charles Rivers Laboratories and incubated in polysterene egg incubators (Hova-bator) at 37°C with a water tray for humidity.
As the water evaporates from the tray, the humidity around the plant is increased.
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