Lane 1 is trypsin-activated Cry1Ac toxin and lane 5 is the M13 helper phage.
Lane 1 Cyt1Aa protoxin; lane 2 M13-Cyt1Aa phage particles; lane 3, M13 helper phage.
The phagemid is rescued as particles by a modified R408 helper phage, deficient in pVIII production.
The peripheral MP neuromodulin displays robustly with packaging by conventional M13-KO7 helphagehage.
Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage.
Furthermore, we show that by using a helper phage (Phaberge) that assembles phage particle preferring the Cry1Ac-pIII fusion protein, display of Cry1Ac was further improved (Fig. 1a).
This study introduces a new genetically engineered M13K07 helper phage displaying multiple copies of a known gold binding peptide on p8 coat proteins.
The libraries were constructed from mice immunized with asialoglycophorin and selected using TF displayed on two different carrier molecules in combination with the proteolytically cleavable helper phage KM13.
The monotopic MP Nogo-66 can also display on the phage surface, if packaged by the modified M13-KO7+ helphagehage.
We compared phagemid particles displaying EGF at high or low valence by rescuing the vector with R408d3 (pIII deleted) or wild-type R408 helper phage, respectively.
We here report a new method of an efficient and direct bacterial expression system for the phagemid-coded Fab proteins without use of the helper phage.
