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This study reports development of enhanced silencing suppressors by engineering the helper component proteinase (HCpro) of Potato virus A (PVA).
(A ) Left: the CaMV transmissible complex comprises the virus particle, composed of capsid protein P4 (yellow), virus-associated protein P3 (blue) and the helper component P2 (red).
This produced a marked granulomatous pneumonia, diffuse infiltrates, and multifocal aggregates of interstitial lymphocytes with a pronounced T helper component and pulmonary in situ lymphocyte proliferation.
Agrobacterium cultures transformed with the tobacco etch potyvirus helper component protein (HC-Pro) silencing suppressor [ 53] were resuspended in infiltration media to the same OD600.
Here we report that the slow-transforming helper component of Friend murine leukaemia virus (Fr-MLV), which produces lymphoid leukaemias in normal mice, induces erythroleukaemia in mice given syngeneic pituitary grafts (SPG).
C57BL (FV-2r) mice were completely resistant to FV; however, those receiving FV and ATS developed late-onset leukaemia histologically characteristic of that produced by the helper component of the FV complex.
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Here we describe the use of naked double-stranded (ds) linear DNA as a way to insert exogenous DNA sequences into chromosomes of mouse hepatocytes in vivo, without helper components such as integrase or transposase.
For example, in the case of potyviruses, the coat protein (CP), an RNA helicase (CI), and a helper component-proteinase (HC-pro) are essential for the movement function [3], [4], [5], [6].
In this study we have explored these effects using previously characterized transgenic tobacco plants which constitutively express (under CaMV 35S promoter) the helper component-proteinase (HC-Pro) derived from a potyviral genome.
Many of the VSRSs mediate also some other essential viral function, e.g. several of them function as viral cell-to-cell or long distance movement proteins, coat proteins, replicases, helper components for viral transmission, proteases, or transcriptional regulators.
One aspect of this paper that was not immediately clear on first reading was the functional relationship between FeatureViewer – which renders annotation layouts into SVG, and the helper components SimpleFeatureRenderer and DASFeatureRenderer.
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