Exact(4)
Total RNA was isolated from heart tissue using the RNeasy kit (Qiagen, Venlo, The Netherlands) according to the manufacturer's protocol.
Total RNA was isolated from heart tissue using the TRIzol isolation method according to the manufacturer's protocol (15996 026, Invirtrogen, USA).
Total RNA was extracted from homogenised heart tissue using the SV total RNA isolation kit (Promega) according to the manufacturer's instructions.
We confirmed the effectiveness of the DDVP exposure by measuring cholinesterase (ChE) activity in extracts from brain and heart tissue using the Ellman method; heart ChE activity is considered to result chiefly from the presence of residual blood in the organ [ 29].
Similar(4)
Total RNA was extracted from homogenized cells and heart tissues using the mirVANA miRNA isolation kit (Ambion, Austin, TX, USA) according to the manufacturer's protocol.
Total RNA was isolated from approximately 100 mg of proximal forelimb or heart tissue using TRIzol® (Invitrogen) according to the manufacturers instructions.
Total RNA was extracted from P7, P28, P53, differentiated cells, and whole mouse heart tissue using TriZol reagent (Invitrogen), according to the manufacturer's instructions.
Recently Ko et al. have shown high expression of a greater than 150 KD protein in the human heart tissue using an antibody based on this cDNA sequence [ 21].
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