Sentence examples for breast tissue using the from inspiring English sources
Total RNA was isolated from homogenized breast tissue using the RNeasy® Tissue Mini Kit (Qiagen) according to the manufacturer's instructions.
Total RNA was isolated from breast tissue using the RNeasy Mini kit (Quiagen, Hilden, Germany), including DNase treatment.
Genomic DNA was extracted from peripheral blood or frozen breast tissue using the QIAGEN DNA Extraction Kit.
Total RNA was extracted from 100 200 mg of breast tissue using the guanidine isothiocyanate/phenol chloroform method (Chomczynski and Sacchi, 1987).
Total mRNA was extracted from 50 to 75 mg of breast tissue using the TRIZOL (Invitrogen, Paisley, UK) method according to manufacturer's protocol.
Brueggemeier et al (1999, 2001) looked at the levels of aromatase (cyp19) and cox-2 gene expression in breast tissue using the semiquantative, reverse transcriptase polymerase chain reaction (RT-PCR) technique.
Finally, we analyzed normal and tumor breast tissues using the Sequencing by Synthesis technique (Illumina Genome Analyzer, Solexa) to simultaneously localize genome-wide copy number alterations and to detect genomic variations such as substitutions and point-deletions and/or insertions in FFPE tissue samples.
Finally, we analyzed normal and tumor breast tissues using the Sequencing by Synthesis (Illumina Genome Analyzer) technology to simultaneously localize genome-wide copy number alterations and to detect genomic variations such as substitutions and point-deletions and/or insertions in FFPE tissue samples.
Total RNA was extracted from different cell lines and normal breast tissues using the RNAeasy minikit (Qiagen).
Cells (normal and malignant) were retrieved from the breast tissues using the following two methods: a) flushing with a 1-cc syringe containing any type of cell culture media; and b) dislodging cells with serrated-end forceps.
Overall, when viewing the breast tissue using confocal imaging, the architectural and cytologic features characteristic of each diagnosis were analogous to those seen using standard histologic processing.
