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Pulses of the headspace sample from the TD chamber were then delivered by a gas sampling system into the test chamber containing the array of cross-reactive nanomaterial-based sensors (Barash et al, 2012; Peled et al, 2013).
Each headspace sample has been recorded for one cycle, which included 5 min of the sensors' baseline response in vacuum, followed by 5 min of VOC exposure, ending with another 5 min in vacuum.
First, to determine if each headspace sample was attractive, repellent or neutral to aphids, we used paired T-tests which tested whether aphids responded to each sample differently from solvent controls in the 4-arm olfactometer bioassay.
In brief, for preparation of calibration curve, 0.1 ml of ethanol standard solution (0.5, 1, 3, 5, 7, or 9 mg/ml in water) and 0.5 ml internal solution (100 μg/ml tert-butyl alcohol in water) were transferred into headspace sample bottles (10 ml volume) and vortexed for at least 30 s, followed by screwing the lids.
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Briefly, polyethylene terephthalate (PET) containers were used to enclose leaves (300 mL sampling volume) and flowers (30 mL sampling volume) for headspace sampling.
This report describes a new platform for headspace sampling technique, i.e. a syringe pump assisted headspace sampler (SPHS).
The preconcentration step was accomplished using an Agilent 7694E Headspace Sampler.
An experimental design was carried out in order to optimize the headspace sampling.
Headspace samples were collected with SPME (solid phase microextraction) and analyzed by a GC MS (gas chromatography mass spectrometry) system.
The headspace sampling has been referenced off-line by qualitative chromatograms of the volatile compounds constituent the odour under test.
For the extraction and collection of accelerants from fire debris, a portable dynamic headspace sampler is used.
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