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The mixture was heated at 60 °C in 20 mL headspace vial with equilibrium time of 10 h.
Homogenized sample of 1 mL was spiked with 100 ng of the internal standard in the 4 mL headspace vial for quantitative analysis.
In this assay, an overnight grown culture was transferred to a 10 mL GC headspace vial (Macherey Nagel, Germany) resulting in a 2 mL culture with an OD600 of 0.1.
The optimized method needs no organic solvents, uses only two grams of sediment on a dry weight basis and involves a unique sample presentation whereby sediment is spread uniformly as a thin layer inside the walls of a glass headspace vial.
To this aim, a weighed amount of essential oil was placed in a headspace vial, after adding a given amount of anisole the vial was sealed and then allowed to stand for 30 min at 0 °C to establish the equilibrium at that temperature.
For solvent measurements, 100 µL of liquid sample were transferred in a 20 mL headspace vial crimped with a Teflon coated septum.
Similar(36)
Duplicate gas samples were stored in headspace vials and analyzed after 24 h.
The ground tea samples (2.0 g) were weighed and placed in 20 mL sealed headspace vials.
The device consists of a specially designed O-ring seal screw cap offering gas-tight seal to commercially available headspace vials.
The analytical performance of the new sampler was evaluated using 22 mL headspace vials with 9 mL water samples spiked with polychlorinated biphenyls (PCBs).
Small (~10 mL) flat bottomed, clear, glass injection vials (so called headspace vials, ~22 mm outer diameter, ~40 mm height) with wide opening (see for example J.G. Finneran Associates, Inc., 310020-2346) whicancan be tightly stoppered.
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