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Such a rapid and simple gain of function screen proves an extremely useful tool for dissecting pathogens displaying functional redundancy of multiple virulence factors and toxins.
Unfortunately, the prettiness of the home screen disappears when you enter almost any application or function screen.
Here we perform a genome-wide gain of function screen of B. pseudomallei strain K96243 to identify loci encoding factors with anti-macrophage activity.
Here we have described a gain of function screen which successfully and rapidly detected over 100 anti-macrophage encoding gene clusters within genomic libraries of B. pseudomallei expressed in recombinant E. coli.
Recently, Kuan-Teh Jeang and co-workers performed a loss of function screen with short-hairpin-RNA (shRNAs) cloned in lentiviral vectors to allow the constitutive expression of the shRNAs in Jurkat T-cells [10].
Here we therefore perform a simple gain of function screen in recombinant Escherichia coli to identify the full list of loci potentially encoding toxins, or other factors, with anti-macrophage activity.
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DNA transposons, RNA interference and CRISPR/Cas9-based systems have been powerful tools for loss-of function screening.
Systematic gain or loss of function screens are discovering more miRNAs playing important roles in ESCs and during reprogramming (Judson et al., 2013; Wang et al., 2013b).
This review focuses on several noninvasive methods that have been developed for pulmonary function screening, which are analyzed from an engineering systems perspective.
To compare the Nexfin cardiac output (CO) with the CO obtained from transthoracic Doppler echocardiography (TTE) during routine cardiac function screening.
Genome-wide loss of function screening in the diploid mammalian genome is hindered by the requirement for homozygosity.
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