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The IFA/ELISA reactive/border-line samples were subsequently analyzed by a focus reduction neutralization test, which revealed low titers (1 80) against SAAV in two samples from a patient with hepatic disease.

In nine patients, hantavirus serotyping was performed by chemiluminescence focus reduction neutralization assays (c-FRNTs), as described recently (13).

In further analysis, neutralization antibodies against SEOV strain SR-11 were detected by focus reduction neutralization test (8 ).

Focus reduction neutralization testing and virus sequencing confirmed that the patient in our study was infected with DOBV-Af.

*HFRS, hemorrhagic fever with renal syndrome; FRNT, focus reduction neutralization test; PUUV, Puumala virus; SAAV, Saaremaa virus; Ig, immunoglobulin; DOBV, Dobrava virus; SEOV, Seoul virus; ND, not done.

Convalescent-phase serum samples were collected from 25 patients, and the causative hantavirus was typed by focus reduction neutralization test (FRNT) as described earlier (7 ).

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Most focus on stress reduction, building on Jon Kabat-Zinn's famed and research-tested Mindfulness-Based Stress Reduction program.

a Neutralization titer was defined as an 80% reduction in the foci reduction neutralization test (FRNT80).

Antiviral activity was determined by viral foci reduction assay and qRT-PCR.

Then the percentage of foci reduction (RF %) compared to negative control was calculated as follows: RF = (C-T) × 100/C.

Results from qRT-PCR supported the findings from viral foci reduction but the inhibition of RNA production was more significant than inhibition of DENV-2 formationation.

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