The total protein was extracted using RIPA protein extraction reagent.
Whole cell lysates of cell lines were extracted with CytoBuster Protein Extraction Reagent (Novagen, Madison, WI).
Total cellular protein was extracted using Cytobuster Protein Extraction Reagent (Merck Millipore; 71009).
Islet protein was extracted using the CytoBuster Protein Extraction Reagent (Novagen, Darmstadt, Germany).
Cells were extracted in M-per mammalian extraction reagent (Pierce) with Complete (Roche) protease inhibitors.
Cells were pelleted and total proteins were extracted using YeastBuster™ Protein Extraction Reagent (Novagen).
Cytoplasmic and nuclear fractions were extracted using NE-PER NuCytoplasmicytoplasmic Extraction Reagent (Pierce, Rockford, IL, USA).
The cytoplasmic extracts (cytosol) were prepared using Cytoplasmic Extraction Reagent (Pierce), according to the manufacturer's instructions.
Total protein was extracted from cell pellets using CytoBuster Protein Extraction Reagent (Merck Millipore, Darmstadt, Germany).
Total proteins of approximately 10 cells were extracted by the M-PER Mammalian Protein Extraction Reagent (Pierce. Rockford, IL).
Nuclear extracts were prepared using the NucBuster nuclear protein extraction reagent (Novagen).
