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Exact(5)
To validate the proteomics findings, we either probed for endogenously expressed proteins or stably transfected a tagged cDNA encoding the putative substrate into a cell culture model.
Non-transgenic mice exhibited no insoluble tau either probed with the HT7 or AT100 antibody (Fig. 3e).
Membranes containing the library of synthetic peptides were either probed immediately or stored at −20°C until needed.
Western blots were either probed with a rabbit anti-FLAG polyclonal antibody (1 10,000 dilution in PBS; Sigma Aldrich, Gillingham, UK), or a horse radish peroxidise-conjugated mouse anti-His6 antibody (1 5000 dilution in PBS; Invitrogen).
Initially, parallel studies were carried out using both thiolated and unthiolated 30-mer DNA-1 sequences (Table 1), which were either probed directly or after thermal pretreatment, as previously described.
Similar(55)
No results of either probe have been announced.
While no one was apprehended in either probe, an Army spokesman expressed satisfaction with the response of those in charge of security.
These spots with mixed oligonucleotide probes display cooperative hybridization signals greater than those that can be achieved by either probe alone.
In addition to visual comparison, the area under the curve (AUC) determined via the three methods was compared as a measure of the similarity between IF obtained via either probe, PET or manual sampling.
MBD5 and MBD6 failed to bind either probe.
Chambers that yielded signals for either probe alone or for both probes were detected and counted.
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