Immunoblots were alternatively probed with antibodies directed against the following dopamine-related molecules; TH (1∶1000, Millipore), DBH (1∶1000, Millipore), DAT (1∶1000, Millipore), D1DR (1∶500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), D2DR (1∶250, Millipore), and vMAT2 (1∶1000, Millipore).
Alternatively, probe the thigh with a meat thermometer.
Alternatively, immunoblots were probed with anti-GAD 65/67 (1∶5000, Sigma-Aldrich, St Louis, MO, USA), anti-parvalbumin (1∶10000, Abcam, Cambridge, UK), anti-MBP (1∶1000, Millipore, Bedford, MA, USA), anti-CNPase (1∶1000, Millipore), anti-NR1 (1∶250, Millipore), anti-NR2A/B (1∶150, Millipore), and anti-β-actin (1∶4000, Millipore) antibodies.
RNA quality affects the probe intensities of each probe by two factors: (i) the probe position with respect to the nominal 3′-end of the transcript, L (or, alternatively, the probe index in the probe set, k) and (ii), the hybridization regime.
Alternatively, for re-probing with phospho-specific antibodies, blots were stripped for 4 5 h at room temperature in 2 M glycine, pH 2.5 with 1 g of SDS for each blot, washed extensively in H2O, blocked for 1 h (TBS+5% BSA), and incubated with the next antibody.
Alternatively, these probes may contain conserved motifs and therefore the probes on the microarray will cross-hybridize to multiple transcripts resulting in the higher signal intensities observed on the microarray for these elements.
Alternatively, scanning probe microscopy and mainly conductive atomic force microscopy (C-AFM) are used to characterize carbon nanofiber embedded in SiO2 to interconnect in integrated circuits[14, 15] and randomly dispersed multi-walled carbon nanofiber polymer nanocomposites (R-CNT-PNCs)[16].
Alternatively, different probe sets might be mapped into the same gene because many of them were designed to detect different splice variants of the same gene.
Alternatively, Applied Biosystems probes were used (Table 2) using the TaqMan FAST Universal mastermix.
Alternatively, the probe could be redesigned to target the complementary strand if it is available in the biological sample.
Alternatively, the probe-stained cells were detached with trypsin-EDTA, washed and fluorescence intensity was measured within 60 min by flow cytometry.
