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The bacterial C.F.U. were estimated by cultivating 0.2 µL of a culture in Postgate E medium (Postgate 1984).

For CM + E medium, pH started to increase gradually from 5.31 to 6.23 after the addition of ethanol.

The indicator strain D. alaskensis was grown for 7 days at 32°C in Postgate E medium.

Biofilms of D. alaskensis were prepared by inoculating circular cover glass slides with a mid-log phase culture (105 cells ml-1) grown in Posgate E medium.

The D. alaskensis growth was detected by observing the blackish color of the medium caused by iron sulfide precipitation in Postgate E medium.

The time kill test at MIC level showed an immediate bactericidal effect, that is, no viable cells were recovered after SRB growth in Postgate E medium.

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Similar(5)

The liver was excised and minced in William's E medium (Life technologies, A12176-01, GraNY Island, NY, USA).

Minimal medium was no-citrate-E medium (NCE) (Davis et al. 1980).

MEP-e: Medium-Energy Particle Experiment Electron Analyzer (Kasahara et al. 2018a).

Each flask was inoculated with 20 mL of sterilized E-medium and ten Lemna aequinoctialis Welv.

The test compounds were dissolved in sterilized E-medium at different concentrations, that is, 5, 50, 500  μg/mL in methanol.

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