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However, it is not successful in cases where ninhydrin has been used in concert with zinc chloride as the developing reagent (Saunders, 1989b).
No developing reagent was used as the components of the crude dye were coloured and the separated bands were analyzed visually.
Stationary phase was silica gel 60 F206, 20 × 10 cm TLC plate (Merck), toluene:chloroform:ethyl acetate (8 8 2) was used as mobile phase and anisaldehyde as a developing reagent.
Following these rinses, Ellman's Reagent, the developing reagent supplied by the manufacturer, was added to the empty wells.
To each well, including the blank, 100 µL of ABTS developing reagent was added and the plates were covered with tinfoil and left to develop for 1 hr.
To quantify syncytia, a developing reagent (60 mM Na2HPO4, 40 mM NaH2PO4, 10 mM KCl, 1 mM MgSO4, 50 mM 2-mercaptoethanol, 0.8 mg/mL ortho-Nitrophenyl-β-galactoside) was added and incubated at room temperature for 2 to 4 h.
Similar(46)
The resulted spots were visualized by spraying different colour developing reagents.
The developing reagents were DAB ZLI-90188, ZSGB-BIO) and VECTOR BLUE SUBSTRATE KIT SK-53000, Vector Laboratories , Inc.
Such supercharged antibodies should prove useful for assays in resource-limited settings and for developing reagents with improved shelf lives.
Nevertheless, a literature survey reveals that different wavelengths (visible range) can be used to monitor the reaction products when using MBTH and Ce(IV) as colour developing reagents.
In all cases, antigen-antibody complexes were detected using appropriate horseradish peroxidase-conjugated secondary antibodies (Dianova: Hamburg, Germany) and these were visualized with enhanced chemiluminescence developing reagents (ECL System: Amersham Biosciences; Poscataway, NJ).
Related(18)
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