It is possible to exploit these natural mechanisms of cellular uptake with rationally designed reagents.
While CIL LC-MS with rationally designed reagents for labeling can provide much enhanced detection and quantification of metabolites, pre-analytical process such as cell harvest and cell lysis is also a critical step in the overall workflow for metabolomic analysis.
SNPs were genotyped using TaqMan genotyping assays (Applied Biosystems) employing Applied Biosystems (AB) designed reagents mixes and oligonucleotide/primer pools, and the 7900 HT Fast Real Time PCR system (Applied Biosystems) according to the manufacturer's instructions.
SBN and EHT designed reagents and protocols and performed experiments.
Two sets of cells were placed in sterile chamber slides overnight at 37°C and then incubated with designed reagents for 18 hours.
His research has focused on designing reagents to clean up the environment, and he co-founded a company, Eichrom Industries, to manufacture a resin that removes toxic metal ions from water.
To better characterize and compare cellular responses to various RNAi reagents in one experimental system, we designed a reagent series in corresponding siRNA, D-siRNA, shRNA and sh-miR forms.
The new reagent was designed to obtain reagent related fragmentation of the whole reagent as well as a side group of the reagent.
Trifluoromethanesulfenamide reagent, initially designed as electrophilic reagent, can be considered as a trifluoromethylthio anion source in presence of tetrabutylammonium iodide.
