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The correct loading was controlled by staining with Ponceau S in each experiment.
In particular, the modification of the sense strand of siRNA 18 resulted in an increased silencing activity probably by inducing the correct loading of antisense strand by RISC.
KIF2a/MCAK depletion also rescued the anaphase timing difference in 2 1 cells compared to 2 2 cells and led to the correct loading of SKAP on kinetochores.
Correct loading of the EGFP transgene cassette into the HAC was confirmed by genomic PCR with a specific pair of primers that diagnose reconstitution of the HPRT gene [ 9].
These procedures do not affect the evaluation of patient's condition from the aspect of the severity of patient's state but are of great importance for the evaluation of the correct loading the nurses with work.
The Smc1 Smc3 heterodimer forms a functional ATPase, whose activity is necessary for correct loading of the cohesin complex onto chromosomes (Arumugam et al, 2003; Weitzer et al, 2003).
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By creating an appropriate data-base of contingencies for training the neural network, the proposed method is able to perform correct load shedding in various loading scenario.
The inverse problem is highly sensitive to measurement noise, and the classical pseudo-inverse method generally fails to find the correct loads.
This will enable necessary adaptations when, for instance, some component of an AaaS instance fails to respond or when the AaaS infrastructure needs to enlarge its computational power for continuously ensuring correct load balancing.
The AGVs would have the ability to arrange themselves within the carriage during transit; this would allow the correct load to be by the exit door when it arrives at its destination and needs to leave the train.
Extreme care is required in the finite element analysis to ensure that the correct load and resistance factors have been applied, particularly when several models are being used and the results are linearly superimposed.
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