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Fig. 6 Immunofluorescence staining of Raji cells for confirmation of binding activity and internalization of scFv apoptin.

For additional independent confirmation of binding, we performed a bandshift assay using two oligos flanking the core OCT4 motif (Fig. 8A).

Additional confirmation of binding specificity was obtained from competition experiments in which the wild-type, but not a mutated, SL3 oligonucleotide effectively competed with probe for L22 binding (compare 100× SL3 with 100× mSL3, Fig. 3B).

This allowed us to retain 48 helicases as RNA helicases either due to the presence of an RNA-binding ortholog in other species or confirmation of binding to RNA in P. falciparum.

Two independent experimental approaches were chosen to confirm Ahr-dependency for a subset of representative genes: direct comparison of the transcriptional response of Ahr-expressing Hepa1c1c7 and mutant tao BpRc1 cells deficient in endogenous Ahr, as well as confirmation of binding of Ahr in the corresponding promoter regions by chromatin immunoprecipitation (ChIP).

Similar(55)

Computational simulation of the interface between polysulphide species and carbon nanotube surface provides first-principle confirmation of improved binding between C and S in the polysulphides as wall-to-wall distance is decreased.

Confirmation of rCR2 binding to human C3d+ cells was demonstrated by incubating the cells with rCR2 followed by secondary anti-HIS-FITC (Figure 5b).

Confirmation of the binding was performed by PCR using mature miR-199a* as a primer and the targeting was performed by luciferase assays.

Further confirmation of the binding interaction demonstrated by Biacore was obtained by gel filtration chromatography using Sephacryl - 300 where the presence of high molecular weight C4BP/sCD154 complexes was detected.

In addition, the PRE profiles are broadly similar to the 1HN/15N chemical shift perturbation profile, with large PREs observed for the N-terminal tail, the loop between helices 1 and 2, and the N-terminal end of helix 3, providing independent confirmation of the binding interface identified by chemical shift mapping.

Therefore, we sought confirmation of the binding selectivity from a complementary technique.

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