Another useful class of mutants is conditional mutants.
They also created conditional mutants, which are key to many research efforts, including the knockout consortium.
Unmarked conditional mutants of four predicted essential genes (lolCDE (PA2988-86), lPA4406A4406), rho (PA5239), and def (PA0019)) were successfully constructed using this recombination system.
Isolated cardiomyocytes from conditional mutants were more susceptible to anthracycline toxicity, demonstrating that ErbB2 signaling in cardiomyocytes is requisite for the prevention of dilated cardiomyopathy (DCM) [14].
The generation of conditional mutants has been an effective approach to studying bacteria and validating drug targets, and mutants of Mycobacteria are no exception.
We have also developed tools for the construction of conditional mutants where the expression of the target gene is regulated by an inducible promoter.
Generation of conditional mutants, light regulation of cyclin-dependent kinase activity, light-based patterning of cell growth, and yeast photography exemplified its versatility.
Here we report our identification of 24 conditional mutants in thirteen different essential C. elegans loci.
The average number of cells per cluster was not significantly different between wild-type and conditional mutants (Fig. 4I).
These conditional mutants will be valuable to investigate the role of specific groups of cholinergic neurons in distinct brain functions.
Based on this notion, our Dlk1 conditional mutants should have elevated Notch signaling, which would suppress MyoD expression[79].
