Exact(1)
In order to estimate completeness of sequence data used to define the HITdb OTUs, sampling from multinomial distribution was performed.
Similar(7)
The completeness of sequencing was assessed by Good's Coverage estimator [60] and estimates of species richness were calculated using the non-parametric estimators ACE (abundance-based coverage estimator [61]) and Chao1 [62] through the program biodiv (D.N. Frank, unpublished. http://www.phyloware.com).com
Most of the genomes used in this study are not complete, therefore, the differences in the degree of coverage most likely reflects the degree of completeness of sequencing of the various genomes.
The completeness of each sequence can vary – some completed sequences are more "complete" than others.
Assuming that all genes are equally and independently likely to be present in the sequenced part of the genome, we estimate the completeness of the sequence as p = f/ F; the complete genome is expected to contain N' = N/ p = NF/ f genes.
In addition there are fields for sequence classification and nomenclature, comments, legacy names, information about the completeness of the sequence, its potential to be a pseudo-gene, and links to records in NCBI's nucleotide and protein databases [ 20].
Sequencing of a simpler coculture system would lead to a higher completeness of genome sequence from species.
The question marks refer to the uncertainty as to the completeness of the sequence (see Results).
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