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Since it is difficult to accurately call individual SNP frequencies when the depth of sequence coverage is low, variant frequencies were averaged across larger regions.
Sequencing nuclear genomes requires sequencing the DNA library many times over, increasing the "depth" of sequence reads at each base.
The depth of sequence reads therefore varied from sample to sample.
The depth of sequence coverage at targeted positions is clearly a key consideration for targeted resquencing.
The RXLR effector with the greatest depth of sequence coverage and highest predicted copy number corresponded to Avh426 (375 bp).
RNA-seq provides a powerful means of measuring gene-expression because the depth of sequence coverage of a transcript should be proportional to its expression level [4].
To further investigate the repetitive nature of the Avr1a and Avr3a loci, trace files from whole genome shotgun sequencing were searched to determine the number of matches, and thus the depth of sequence coverage, to particular sequence intervals.
It is unlikely that the difference is due to the depth of sequence collection.
Error rates in sequence data vary considerably, depending on the depth of sequence coverage.
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An average depth of sequence coverage of 170 and a median coverage of 140 was attained for the main captured exome run (HiSeq-1).
The 39× average depth of sequence coverage of the genome was achieved using predominantly data generated with the Roche 454 platform, supplemented with Illumina GAII and ABI/SOLiD data, and was assembled de novo into ∼3200 sequencing scaffolds with an N50 of 1.3 Mb.
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