We used confocal imaging of dorsolateral striatal sections stained for GFP and TH, collected image stacks, and measured the density of GFP-positive fibers using a threshold approach as described in the Methods.
Detect the edge areas of the collected image to obtain the binary image.
The decision layer uses one-against-rest support vector machines (SVMs) to establish classifiers for applying the collected image characteristics to the calculation of insertion motions.
The MTF value of the collected image is calculated, which is compared with the MTF values of the prearranged focal images, and the judgment of the focusing error is completed.
The MTF values of the optical system do not vary with the collected image changes [18], so the MTF values at the Nyquist frequency can be gotten via testing the focusing optical system in advance.
To investigate responses to a sharp gradient we positioned the interface between the streams of DFA and DEX over the center of an explant for 120 minutes (Figure 2A) and collected image stacks at 60 minute intervals using confocal microscopy.
