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Furthermore, polymorphism tests of 15 wild P. tomentosa ecotypes indicated that exonic SSRs contained less allelic variability than did non-coding SSRs (Supplementary Table S7B), which reflects the higher selection pressure on the coding portion of the genome.
Once they're recruited, experimental biologists perform a genomic profile of each patient, sequencing the exomes the coding portion of the genomes of individual tumors.
The analysis of rare variants of the genes was performed by sequencing of the coding portion of the gene and 5'-untranslated region (5'UTR) with the Sanger method.
It was clear from our previous attempts to map gurken's RLEs that one or more such elements are located in the protein coding portion of the gene [21].
While we have not tested this possibility, it is noteworthy that previous studies have indicated that the GLS "retains" AD localization, even when located downstream of the protein coding portion of a GFP reporter transcript [20].
The outlay of time and labor to validate the disease association concerning a variant of uncertain significance (VUS) within the coding portion of a gene can be daunting and cost prohibitive [3], [4].
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The potential protein-coding portion of BRCA2 999del5 was cloned into the pIND SP1)/V5-His vector and expressed in COS7 cells.
Sequences in the non-coding portion were evaluated for coding potential using CPAT (http://lilab.research.bcm.edu/cpat/index.php).edu/cpat/index.php
The non-coding portion immediately upstream of the coding region presumably includes the coding exon acceptor site, as well as translation regulatory signals.
In the second step, the entire protein-coding portion of the mitochondrial genome (13 complete coding sequences) was collected for 107 mammalian species, including sequences from all major mammalian lineages, from GenBank (Supplementary Table S1).
How does the non-coding portion of the genome contribute to the regulation of genome architecture?
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