Thus, CDSs only covered on the coding strand have a non-coding coverage ratio equal to 0, while higher values reveal the presence of reads that also map the non-coding strand.
By including biological replicates and mapping TSSs in addition to transcripts, our study shows that pervasive transcription in P. acnes stems as much from transcription of the coding strand as the non-coding strand of annotated genes.
That is, the stretch of DNA switches orientation what was the coding strand is now the non-coding strand, and vice versa.
Moreover, this sequence was present in the non-coding strand of all alleles at coding strand position 37 42 (Table 2).
a) forward: coding strand contains 5'-amino modification; reverse: non-coding strand contains 5'-amino modification.
amotif ± 1 bp b motif in non-coding strand corresponding to these bases in the coding strand.
Genes with a G/C tract within an intron (intragenic tracts) had on average 2.05 SAGE tags (2.21 tags if on the non-coding strand and 1.77 tags if on the coding strand).
Whether one starts from the 5' end of the coding strand, or from the 5' end of the non-coding strand, there is a strong purine skew near the telomere.
