Exact(2)
The same analysis conducted on eight Treg clones, with unknown antigen specificity, shows that TCRs shared with effector CD4+ T cells (receptor sequences depicted in black and labeled with N) constitute 18.2% and 3.7% of all Treg cells in tumors and tumor draining lymph nodes respectively and are not found in the control lymph nodes.
In order to analyze the relative reactivity of newly selected anti-CEA scFv mutants (the presence of TGA or TAG triplets in some phage clones with unknown suppression levels of stop codons prevents a correct analysis of antibody reactivity), several mutant scFv genes from E1, E2, E3, E4, E6, E8, E13, E14, E15 and E16 clones were amplified with KM148-KM253 and KM252-KM143 primers.
Similar(6)
To evaluate the system, 55 cDNA clones with predominantly unknown function were selected from a mouse testis cDNA-library.
Clusters E and F included genes encoding Terf1-interacting nuclear factor 2, tissue inhibitor of metalloproteinase 1, makorin, and DNA clone 4833424O15 with unknown function (Table 2 and Fig. 5).
ADAMTS17 is another recently cloned ADAMTS family member with unknown function and this is the first report of its expression in synovium.
An added complication is that a Chinese company has reportedly begun manufacturing a clone of the iPhone X — with unknown facial recognition capabilities.
One hundred and six clones gave no results or matched sequences with unknown functions.
Since the frequency of antigen-specific B cell clones was unknown, we started with micro-cell cultures ranging from 0.6 to 640 cells per well.
Related(1)
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com