Sentence examples for cloned vector from inspiring English sources

The cloned vector was then transfected into primary human fibroblasts.

The cloned vector was transfected into primary human fibroblasts.

The cloned vector was transformed into bacterial DH10Bac competent cells for making recombinant bacmid.

The cloned vector was transfected into primary human fibroblasts resulting in suppression of collagen expression [ 8].

The cloned vector, pENTR/KW/EVBP, was used for the transformation of E. coli cells (One Shot TOP10 Chemically Competent Cell, Invitrogen Corp., Carlsbad, CA, USA).

The resulting cloned vector pBJ1000 (50 ng/µl) was coinjected with transformation markers unc-30 (100 ng/µl of pSC11) and ajm-1:: gfp (50 ng/µl of pJS191) into dpy-20 (e1282 ) unc-30 (e191 ); huIs1 worms using standard injection protocols (Mello and Fire 1995), and one line was selected for integration using gamma irradiation (3850 RADs).

The indicated constructs were cloned into the TA-cloning site of cloning vector pT7Blue.

Cloning vector pJET1.2/blunt (Thermo Fisher Scientific, Canada) and E. coli BL21 DE3 pLysS contained ampicillin- and chloramphenicol-resistant genes, respectively.

Cloning vector pDrive was obtained from Qiagen and modified as previously described (Rodriguez-Cabello et al. 2012).

PCR products were subcloned into the pCR8/GW/TOPO TA cloning vector (Invitrogen, Carlsbad, CA, USA).

Gel purified PCR product was cloned in TOPO TA cloning vector (3.9 kb) and transformed into TOPOF' cells.