Sentence examples for cloned construct from inspiring English sources
The model is described and characterized in details in reference [22], where it was verified that the cloned construct MOP-EGFP exhibits properties similar to the native MOP that the construct inserted in the plasma membrane, is sensitive and selective to its specific ligands and coupled to the cellular trafficking machinery (Fig. 1A).
The final cloned construct contains the initiator Met codon and the 67 terminal amino acids (from residue 837 to 903) of human mt LeuRS.
AID cDNA was PCR-amplified from C57BL/6 mice (primers: forward 5′-TTCTGTGAAGACCGCAAGGCT-3′; reverse 5′-CCCTTCCCAGGCTTTGAAA-3′), cloned into the pENTR/D-TOPO Gateway vector (Invitrogen), and subsequently recombined into the Rosa 26 targeting vector, in which the cloned construct is preceded by a loxP-flanked transcriptional stop cassette and followed by an internal ribosomal entry site and GFP.
The integrity of the cloned construct was checked by nucleotide sequencing.
On 5 April, the sequence of the cloned construct was verified.
After amplification, the PCR product was ligated into the expression vector pCRT7/NT TOPO (Invitrogen), and the sequence of the cloned construct was confirmed by DNA sequencing.
S.N.K. cloned constructs and worked on the initial conditions for NMR and ITC experiments.
All cloned constructs were fully sequenced.
The cloned constructs were confirmed by sequencing.
The cloned constructs were used for in vitro transfection assays.
Cloned constructs were transformed into chemically competent E. coli Top10 F- cells (Invitrogen Life Technologies, Carbsbad, CA).
