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Ishimura et al. ([1991]) showed a faster and improved healing of avascular meniscal defects in a rabbit model by using bone marrow fibrin clot constructs compared to fibrin clot alone (Ishimura et al. [1991]).
To overcome these drawbacks, we have developed a new method for creating a tissue-engineered vascular graft by using bone marrow cells, which can be obtained easily and used immediately, without cell culture.
Completing the tissue engineering triangle of cells, signal, and matrix by using bone marrow aspirate concentrates, recombinant human bone morphogenetic protein-2/acellular collagen sponge, and crushed cancellous mineralized allogeneic bone predictably regenerates large volumes of histologically and functionally useful bone in jaw reconstruction.
The role of the epithelium was underlined by a comparable approach for the urethral reconstruction by using bone marrow MSCs and SMCs into a bladder acellular matrix [ 171].
An improvement in experimental neuropathic pain treatment was also obtained using other types of cells isolated from bone marrow and in particular by using bone marrow derived mononuclear cells.
We did not try to mobilize EPCs from bone marrow, i.e. by means of the granulocyte colony-stimulating factor (G-CSF), because the yield of these cells in peripheral blood is, in any case, lower than that registered by using bone marrow itself [ 13, 35].
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The genetic evidence obtained by using bone marrow-derived macrophages from Notch 1/2/3 knockout mice or Notch 1 or Notch 3 knockout mice, however, solidify the finding that Notch negatively regulates osteo-clastogenesis [ 51].
More importantly, we could substantially reconstitute the cross-priming capacity of DCs that had engulfed infected neutrophils by using antibodies to block engagement of Mer, or by using bone marrow-derived DCs prepared from Mer−/− mice.
They maintain, instead, an intact self-renewal potential, as shown by the transplantation experiments using bone marrow from mice treated three consecutive times with INFα.
The aim of this study was to evaluate the angiogenic capacity and proteolytic mechanism of coculture using human amniotic mesenchymal stem cells (hAMSCs) with human umbilical vein endothelial cells (HUVECs) in vivo and in vitro by comparing to those of coculture using bone marrow mesenchymal stem cells with HUVEC.
In conclusion, this study demonstrates that the application of long-term hydrostatic pressure can be used to improve the functional properties of cartilaginous tissues engineered using bone marrow derived MSCs by enhancing collagen and GAG accumulation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com