Sentence examples for by sequential washes in from inspiring English sources

Immune complexes were precipitated with protein G-Sepharose beads followed by sequential washes in IP buffer.

Five micrometer thick tissue sections mounted on silanised slides were deparaffinised in xylene followed by sequential washes in graded ethanol to phosphate-buffered saline (PBS).

Slides were then rehydrated by sequential washes in ethanol (100%, 95%and70%0%, 1 × 5 min ., deionized water (2 × 2 min).

This process was followed by sequential washes in alcohol solutions of decreasing concentrations to gradually remove the alcohol and rehydrate the sample.

Slides were washed with 2 × SSC, 0.2% SDS for five minutes, followed by sequential washes in 1 × SSC for three minutes, 0.5 × SSC for two minutes, 1 × SSC for one minute and 0.05 × SSC for 15 seconds.

Following a 30-min wash in distilled water, samples were dehydrated by sequential washes in increasing concentrations of acetone for 1 h each (50, 70, 90%), followed by three washes in acetone (100%) for 1 h each.

The labeled miRNA were hybridized to mouse miRNA 8 × 15K microarrays in a rotating hybridization oven at 10 rpm for 20 h at 55 °C followed by sequential washing in Agilent GE Wash Buffer 1 with Triton X-102 and Agilent GE Wash Buffer 2 with Triton X-102.

Next, 100 μl of probe was injected onto the slide and hybridised with agitation at 50°C for 16 hours, followed by sequential washing in four cycles of 2× SSC, 0.1% SDS, 2 cycles of 1× SSC, 0.1% SDS, and 0.1× SSC, 0.2% SDS at 40°C, 2 cycles of 0.1× SSC at 23°C, followed by a final cleaning cycle of ultrapure water before drying.

Biotinylated single strand DNA was obtained by incubating the immobilized PCR products in 0.5 mol/L NaOH, followed by two sequential washes in 10 mmol/L Tris-acetate, pH 7.6.

Bacteria were made electrocompetent by sequential washes with ice-cold sterile 10% glycerol, and transformed with approximately 500 ng of each purified PCR product.

Overnight hybridization was followed by sequential washes with wash buffer 1 (50% formamide, 5× SSC, pH 4.5 and 1% SDS in PBT) followed by buffer 2 (50% formamide, 2× SSC, pH 4.5, and 0.1%Tween-20Tween-20) at 63°C to remove unbound probe.