Sentence examples for by multiple washes in from inspiring English sources

Slides were then washed twice with 70% formamide, 10 mM Tris HCl pH 7.0 7.5, 0.1% BSA followed by multiple washes in PBS.

Excess paraffin was removed by multiple washes in xlyene.

Finally, all the CNTs-supported NPs were extracted from [BMIm][BF4] using high-speed centrifugation and decantation, followed by multiple washing in acetone aided by bath ultrasonication.

Unbound protein was removed by multiple washes with binding buffer and bound protein was eluted in 100 µl elution buffer (10 mM glutathione, 50 mM Trizma-HCl, pH 7.4) at room temperature for 30 minutes.

Post-infection samples were washed extensively and partially attached and non-ingested beads/bacteria are removed by trypsin treatment followed by multiple washes.

The troughs were filled with lipids by immersing the test device in a solution of liposomes, 1,2-dihexadecanoyl sn-glycero-3-phosphocholine [PC 16:0/16:0)], and cholesterol (Avanti Polar Lipids, USA), followed by multiple washes to remove lipids deposited on the PEG.

Following 2 hr infection, the cells were removed of remaining viruses by multiple washes with fresh medium.

Wash the affected region of hair two to three times with the dish soap, followed by multiple washes with shampoo.

Fusion was initiated by addition of 40% PEG (1500) in PBS for 50 sec, followed by multiple washing steps with PBS and further incubation in medium at 37°C for the indicated time periods.

After 4 h of incubation at 37°C in modified Medium199, non-phagocytosed parasites were removed by multiple washing steps with PBS and incubation was continued for another 20 or 44 h in IMDM at 37°C and 9% CO2.

Following multiple washes, samples were mounted in Vectashield mounting medium (Vector laboratories, Burlingame, CA).