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Average particle sizes were estimated using SEM micrographs by counting a minimum of 100 particles, obtaining values of 35 ± 17 nm for anatase and 47 ± 18 nm for rutile.
Percentages of C. pneumoniae- LPS positivity was calculated by counting a minimum of 200 cells/slide.
Percent parasitemia was estimated by counting a minimum of 1000 erythrocytes and calculated using the following formula: (# infected erythrocytes/total # erythrocytes) ×100.
F4/80 staining was quantified by counting a minimum of three 400× fields from each tumor.
Quantification of additional images was performed by counting a minimum of nine fields at × 20 magnification.
Quantification of the number of V5-positive, or NF-aggregate positive cells was performed manually by counting a minimum of 100 cells per experiment for each cell line.
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GFP and SPIO containing cells were determined by manually counting a minimum of 100 cells per sample.
e., visible MWCNT inclusions in Mφ) were determined by bright-field microscopy, counting a minimum of 500 cells from a 100 mL cytospin slide stained with Diff Qwik (Dade Behring Inc., Newark, DE, USA).
All morphology counts were performed at least twice by two or more investigators counting a minimum of 300 cells each.
Cells were then washed and resuspended in PBS and analysed by flow cytometry on a FACS-Calibur (Becton Dickinson Biosciences) counting a minimum of 104 cells per sample.
It should be possible for local haematocrit data to be extracted from our sequences, for example by counting intensity minima passing each point along a capillary.
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