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Cross-linking can be detected readily by a mobility shift on SDS PAGE.
Activation of autophagy triggers LC3-I lipidation resulting in the conversion of LC3-I to LC3-II, which is measured by a mobility shift on SDS-PAGE [27].
7E10 recognizes GRASP65 regardless of its phosphorylation, though phospho-GRASP65 can be detected by a mobility shift of the protein.
This process can be followed by the appearance of LC3 puncta or ring-like structures and by a mobility shift from LC3I to LC3II by SDS PAGE.
Similar(56)
We found that 100, 300 and 1000 ng/ml of Wnt5a induced dishevelled-2 (Dvl2) phosphorylation (visible by Western blot as a mobility shift of the protein) in a dose-dependent manner (Fig. 2A').
Phosphorylation can usually be detected as a mobility shift by SDS-PAGE when epitope tagged versions of Chk1 are detected with antibody to the tag.
We conclude that Exo1 is modified after telomere uncapping and this modification is associated with a mobility shift detectable by western blot.
To help deconvolute the effects of phosphorylation and protein−protein interactions on ssDNA binding affinity, we measured the relative affinity of RPA and pRPA for oligonucleotides by a gel mobility shift assay.
Results from bioinformatics analysis further indicate that the mechanism of the rs2027432 polymorphism's affecting promoter activity might be due to its effect on the binding of transcription factor MyoD, which might be further confirmed by a electrophoretic mobility shift assay.
In another report, the association of a GST-YU2B″ with human SLIV by an electrophoretic mobility shift assay could not be detected, although the protein did bind to human U1 SLII.
While we did not attempt to detect the binding of the transposase to DNA by an electrophoretic mobility shift assay, we analyzed complex formation by analytical size-exclusion chromatography.
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