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Stop codons that would be generated by a frame shift were located downstream of each target sequence.
The ORF 1 codes for the MP, ORF 2a for the poly protein 2a, and ORF 2b for the RNA dependent RNA polymerase (RdRp) that is translated by a frame shift mechanism to yield poly protein 2ab.
Downstream of xcsN in Xac are four genes (five ORFs, since the second gene is split by a frame shift), including another TBDR gene, that are absent from Xav and the X. campestris strains.
In addition, the genes encoding the RtxA secretion system (Y11_10141-10171) are interrupted by a frame shift in O 3/4, raising the question of functional RtxA export.
As evidenced previously [ 4], incorrect annotation of the ORF is caused by a frame shift in the sequence presumably due to a missing base in the genome sequencing.
The smallest predicted protein, SlTCP27, is probably truncated by a frame shift mutation since sequence homology with Arabidopsis TCP20 extends well beyond the stop codon.
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In some cases, AS can truncate the normal protein product by causing a frame shift [ 12].
To reconstruct the original phenotype, the plasmid containing the transposon flanked by genomic DNA isolated from 2A01 was reinserted into the genome of PCC 7942 after inactivating the transposase by introducing a frame shift mutation.
After the Cre recombinase-dependent excision of the exonic sequences flanked by loxP sites, a frame shift occurs that should prevent the production of normal CSGalNAcT1 enzyme.
Removal of exon 2 by Cre recombination invokes a frame shift in the mRNA resulting in a premature stop codon and a knock-out allele termed ' FC'.
MAVS 1a and 1b, due to a frame shift by exon deletion, encode 131 and 124 aa residues, respectively.
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