Sentence examples for buffer and solubilized in from inspiring English sources

The beads were washed three times with lysis buffer and solubilized in 15 µl SDS sample buffer.

Nuclei were washed with hypotonic buffer and solubilized in Laemmli sample buffer.

The centrifugation pellet was resuspended in 1 ml HEPES-EDTA buffer and solubilized in 0.6% v/v SurfactAmps Brij58 (Pierce Endogen, Rockford, IL, USA) for 30 minutes at room temperature.

Inclusion bodies were washed with extraction buffer, and solubilized in urea-lysis buffer (10 mM Tris, pH 7.9, 750 mM NaCl, 5 M urea, 0.1% Triton-x-100, 20 mM imidazole) by sonication.

This pellet was washed with HOMO buffer and solubilized in 10 μL/oocyte of RIPA buffer (1% Triton X-100, 1% Na deoxycholate, 0.1% SDS, and 20 mM Tris/HCl, pH 7.3).

At indicated time points, the cells were rapidly washed with ice-cold phosphate-buffered saline and solubilized in hypotonic buffer A (10 mM HEPES-KOH, pH 7.9, 1.5 mM MgCl2, 10 mM KCl, 0.5 mM dithiothreitol, 0.2 mM phenylmethylsulphonyl fluoride, 1 mM sodium orthovanadate, 10 μg/ml leupeptin, 25 μg/ml aprotinin, 1 mM NaF and 0.1 mM EGTA).

Cells were incubated in the presence of 10 ng/mL PDGF or IFN-γ with or without DS-SILY20 for 10 or 60 min prior to washing with ice cold tris buffered saline (TBS) and solubilized in lysis buffer (150 mM NaCl, 20 mM Tris, 1 mM EDTA, 1 mM EGTA, 1% Triton-X-100, plus protease inhibitors and phosphatase inhibitors).

Plates were rinsed twice in ice-cold phosphate buffered saline (PBS) and solubilized in lysis buffer (1% NP-40, 50 mM Tris-HCl, 150 mM NaCl, 2 mM EDTA, 50 mg/ml leupeptin, 0.5% aprotinin, 1 mM sodium orthovanadate, 1 mM PMSF).

For immunoprecipitations, cells were washed in ice-cold phosphate buffered saline (PBS) and solubilized in 1% Brij 96 V lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5 mM EGTA, 1 mM MgCl2), containing 1 mM Na3VO4, 50 mM NaF, 10 mM Na4P2O7 and a cocktail of protease inhibitors [35].

Cells were washed twice with ice-cold phosphate buffered saline (PBS) and solubilized in 0.1 N NaOH.

Transfected 293T cells were washed with phosphate-buffered saline (PBS Fisherer) and solubilized in IP buffer (PBS, pH 7.4, 2% IGEPAL (Sigma), supplemented, prior to use, with Protease Inhibitor Cocktail for use with mammalian cell and tissue extracts (Sigma)).