Sentence examples for buffer and homogenized in from inspiring English sources

After being washed with PBS, the cell pellets were resuspended in 1.0 ml cytoplasmic extraction buffer and homogenized in a precooling Dounce homogenizer (Active Motif, Carlsbad, CA, USA), followed by vigorous shock for 15 s and incubation in ice for 15 min.

Frozen tumor tissue aliquots were suspended in the same lysis buffer and homogenized in a 1 ml glass homogenizer.

The individual sediments were pooled, resuspended in the same buffer and homogenized in a tightly fitting Teflon glass homogenizer surrounded by an ice bath.

HEK293T or RKO cells were harvested, washed twice with DPBS buffer, and homogenized in buffer A (0.3 M sucrose, 10 mM Tris-HCl pH 8.0, 3 mM CaCl2, 2.5 mM Magnesium acetate, 0.25% Triton X-100, 0.1 mM EDTA, 2 mM DTT, Complete™ proteinase inhibitors (Roche)) in a douncer homogeniser.

Tissue samples were suspended in 1 mL of cold homogenizing buffer and homogenized in an ice-cold grinder.

Then, samples were suspended in 1 mL of cold homogenizing buffer and homogenized in an ice-cold grinder.

The frozen liver sample was minced in ice cold phosphate buffered saline and homogenized in cell lysis buffer (Cell Signaling Technology Inc., Danvers, MA).

Hearts from WT and ecSOD Tg mice were isolated, frozen, pulverized, and homogenized in buffer and homogenates prepared and processed for PAGE and Western analysis as described previously [ 25].

The pellet was resuspended in lysis buffer containing 1% triton and homogenized in a dounce homogenizer.

The lumen was first flushed with EDTA PBS buffer, and then, tissue was collected and homogenized in PBS buffer and analyzed as unit per milligram protein (a).

The tissues were then rinsed with 0.01 M phosphate buffer solution (PBS) briefly and homogenized in liquid nitrogen using mortar and pestle.