Sentence examples for buffer and loaded in from inspiring English sources

Proteins were solubilized in LDS buffer and loaded in a 4 12% NU-PAGE gel with MES buffer using a NOVEX Powereasy500 apparatus.

The samples were diluted in non-reducing Laemmli sample buffer and loaded in equal amounts on a 7% gelatin-PAGE gel.

Baculovirus produced E2 protein, kindly provided by the Italian Reference Centre for Classical Swine Fever (Istituto Zooprofilattico dell'Umbria e delle Marche), was resuspended in sample buffer and loaded in 12% SDS-PAGE and blotted to Hybond C+ membrane (Amersham).

The reaction sample was then incubated for 20 min at 37°C and the reactions stopped by addition of SDS-PAGE loading buffer and loaded in SDS-PAGE gels.

For protein loading control, the 10 μl of the DDM-treated mitochondrial preparation was mixed to an equal amount of Laemmli sample buffer and loaded in an SDS PAGE.

The products were diluted 1 2 in sucrose buffer and loaded in a partially denaturing MDE® gel (Cambrex, UK) at constant power of 7 W during different time periods depending on the size of the fragment.

To infer molecular weights, 1 μl of each sample was mixed with 6× orange loading buffer (NEB) and loaded in a 2% agarose gel using 3 μl of 50bp ladder (Fermentas), 1× SB buffer (728 mM NaOH, 200 mM boric acid) and run at 150 volts for an hour.

The libraries were diluted in buffer EB (QIAGEN) to 10 nM, denaturated with 2 N NaOH to a final DNA concentration of 0.5 nM, diluted to 2 4 pM with pre-chilled Hybridization buffer (Illumina) and loaded in individual lanes into a 1.0 mm flowcell together with a single lane of a 2 pM PhiX control library (Illumina).

Total protein (30 50 mg) was mixed with Laemmli buffer containing DTT and loaded in Novex 4 12% precast gels and ran in an X-Cell running system at 120 V for 1.5 h.

Liposomes were diluted ∼10-fold in reconstitution buffer, and loaded into the flow chamber, where they spontaneously adhere to the glass surface.

In brief, proteins extracted from the 2700 MII oocytes were dissolved in SDS-PAGE loading buffer, boiled for 5 min, and loaded in a single lane on a 1-mm-thick 10% polyacrylamide gel.