Sentence examples for buffer and added in from inspiring English sources

200µL plasma was diluted with 400µl binding buffer and added in triplicate to the 96-well plate.

Female plasma samples, nestling plasma samples and the chloroform extracted yolk fraction were diluted 1∶1,000 in sample buffer and added, in duplicate, at a volume of 100 µL/well.

Concentrated OPP preparation was dissolved and diluted serially with buffer and added in cumulatively, directly to the bath.

Retinal homogenates of control and cholesterol-fed rabbits were diluted in reaction buffer and added into 96 well plates.

Afterwards, 200  μL of Dig-labeled A10-2 was diluted into different concentrations (0.078 40 ng/ μL) in binding buffer and added into each well.

Lysis buffer was freshly prepared and added in 6-well plates (100  μL/well) on ice, which were then incubated for 10 min.

Animal serum was diluted 1∶100 in 1% BSA blocking buffer and added to the wells in duplicate.

SDS was omitted from this buffer and only added in the subsequent step because excessive foaming was observed when trying to resuspend the cells in its presence.

CD31 enzyme-linked immunosorbent assay (ELISA) substrate was prepared by dissolving p-nitrophenol phosphate in Tris buffer and was added in accordance with the manufacturer's instructions.

Soluble exE-selectin/Fc and Fc-only (10 µg/ml each) were dissolved in blocking buffer and added to the cell culture dishes.

To label actin structures, Alexa Fluor 568 labeled phalloidin (Invitrogen, A12380) was diluted at 1∶400 in blocking buffer and added to secondary antibody solutions.