Sentence examples for buffer and incubating for from inspiring English sources

After washing in a Tris-buffered saline with 0.1% Tween (TBS-T) buffer and incubating for 45 min with a secondary antibody (horseradish peroxidase-conjugated goat anti-rabbit IgG; 1 : 5000), positively stained bands were detected by a chemiluminescent blot assay with the ECL Plus western blot reagent.

Microarrays were pre-hybridized by adding 130 μL of Hybridization buffer and incubating for 10 minutes at 45°C and 60 rpm.

Samples were prepared by adding an equal volume of 2 × SDS sample buffer and incubating for 10 min in a boiling water bath.

For LC3B analysis, total lysates were obtained by resuspending washed cellular pellets in 1× Laemmli buffer and incubating for 10 min at 95°C, in continuous agitation.

Amyloid fibril/lipid mixtures were prepared by diluting fibril and liposome stock solutions ¼ in liposomes buffer and incubating for 1 16 h at room temperature, shaking at 700 r.p.m.

Finally, the sample was eluted from the Dynabeads® by adding 25 μL of elution buffer and incubating for 10 min at room temperature before pelleting in a magnetic rack.

Subsequently, the gel was washed twice with Triton-X 2.5%, once with the incubation buffer and incubated for 24 h at 28 °C in the same buffer (Tris HCl 0.05 M, pH 7.5, containing CaCl2 2 mM and NaN3 0.02%).

Membranes were washed with incubation buffer and incubated for 1 hour with horseradish peroxidase-coupled goat polyclonal anti-rabbit IgG (dilution 1∶3000).

Samples were diluted in assay buffer and incubated for one hour, followed by incubation for one hour with tracer antibody.

After incubation, the cells were washed in stain buffer and incubated for 30 min at 4°C with a biotinylated sheep anti-human antibody (GE Healthcare/Amersham; 1 100).

The membrane parts were washed thoroughly with TBST buffer and incubated for 1 h with the corresponding horseradish peroxidase-conjugated IgGs (Santa Cruz Biotechnology Inc). in 3% BSA/TBST.