Sentence examples for buffer and boiling for from inspiring English sources
The pellets were resuspended in 100 µL TBS (Tris-buffered saline) buffer and lysed by addition of 100 µL SDS-PAGE loading buffer and boiling for 10 min. 3 × 20 µL samples of BW25113 lysate were resolved by SDS-PAGE along with purified CpoB standards (0, 1, 2, 4, 8, and 16 ng) loaded in 20 µL of BW25113 Δ cpoB lysate.
Reactions were stopped by addition of reducing SDS sample buffer and boiling for 5 min.
The reaction was stopped by the addition of 4× protein loading buffer and boiling for 5 min.
Reactions were incubated for 1 h at 30 °C, then stopped by addition of reducing SDS sample buffer and boiling for 5 min.
Reactions were terminated by the addition of SDS sample loading buffer and boiling for 5 min.
After incubation in citrate buffer and boiling for heat-induced antigen retrieval, samples were blocked with goat sera and subsequently incubated with primary anti-nitrotyrosine (Millipore, Schwalbach, Germany) or anti-CSE antibodies (Abnova, Taipei City, Taiwan).
Briefly, cells were washed twice in phosphate-buffered saline (PBS) and cell pellets were lysed in electrophoresis buffer and boiled for 10 min.
All reactions were stopped by adding 5 × SDS loading buffer and boiled for 10 min at 95 °C for WB analysis21.
The total of 30 μg/mL of cell lysates were resuspended in Laemmli loading loading buffer, and boiled for 10 min at 95 °C.
