In this study, the number of paraventricular organ-accompanying neurons is counted in adult brain sections stained with anti-tyrosine hydroxylase antibodies.
To quantify neurodegeneration in the cell body and neuropil, images of the 5 µm brain sections stained with hematoxylin and eosin and were captured using bright-field microscopy.
Infarct volume was measured at 48 h in 1 mm-thick coronal brain sections stained with 1% solution of 2,3,5- triphenyltetrazolium chloride (TTC).
After brain slices exposure to autoradiographic films during 15 days, as described in the "Methods" section, films were developed and brain sections stained with cresyl violet.
Moreover, brain sections stained with WNV envelop antibody revealed absence of infected cells in mice transferred with CD11b cells from si6 treated mice, compared to that from the control siLuc treated mice (Fig. 3f).
To investigate the morphological factors relating to behavioral changes and the decrease in the phoshorylation levels of Akt and GSK3β in cortex, we first analyzed brain sections stained with cresyl violet.
