After 48 hours of protein exposure, increased signal was found in all brain sections compared to control, consistent with increased BBB permeability after initial ischemia in these brain regions.
We observed that uPAR and phospho FAK (Y397) staining were high in the irradiated brain sections compared to control brain sections (Fig. S3).
Immunolabelling for the majority of complement antibodies was similar to but significantly lower in the spinal cord sections compared with brain sections, except for the anaphylatoxin receptors in which immunolabelled cells were observed more frequently in cord (data not shown).
There are now 17 sections compared with the original three of Mammals, Birds and Other Animals.
The draft is short and vague, having only 31 sections compared with neighboring Newport Beach's 105.
Importantly, we note that there is a very high level of auto-fluorescence in brain sections as compared with other tissues, which is perhaps why this fluorescent autophagy reporter has not been used extensively to study autophagy in the brain in prior studies (despite widespread use in other tissues).
Based on the observations of increased p47phox immunoreactivity and activated microglial cells in striatum after I/R, we further examined phospho-ERK1/2 expression in the ischemic brain sections and compared immunoreactivity with the groups supplemented with Sutherlandia and elderberry diets.
Abundant immunoreactive IBA1-positive microglia were detected in 75 d PrPsc and 115 d PrPsc+RADA brain sections as compared to minimal detection in sections from animals inoculated with PrPc+RADA (Fig. 5B; bottom panels).
Animals that received fluoxetine demonstrated a 52% increase in the total number of brain metastases compared with control: fluoxetine (n = 11), 35.54 ± 3.90 vs. control (n = 12), 23.33 ± 2.46 tumors/section, p = 0.02.
