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Independent analysis was performed by two blinded observers using a Leica DMRBE microscope equipped with a Cy3 and 4',6-diamidino-2-phenylindole (DAPI) filters.
To detect de- and remyelination in the corpus callosum myelin stained sections for LFB, MBP, MOG, and PLP were scored by three blinded observers using a scale from 0 (complete demyelination) to 3 (normal myelin).
Slides were examined by three independent, blinded observers using a Leica DMIRE2 microscope (Leica DMIRE2, CCD: DFC 420FX, Germany).
As indictated in Materials and methods, stained sections were scored by two blinded observers using a semiquantitative scale from 0 (lowest expression) to 3 (highest level of expression).
Pathology scores were evaluated for each tissue section by three blinded observers using a modified Mankin scoring system, as described [ 27].
After the GPs' performance had been videotaped, it was evaluated by two blinded observers using a checklist to score the presence of key features of the EAP.
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Per treatment 38 nuclei were chosen in a random fashion by a blinded observer using a Cohu high-performance charge-coupled device camera and a computer monitor.
Slides were imaged by a blinded observer using a custom-built multi-photon microscope.
For quantitative analysis, slides were coded and then images captured by a blinded observer using a Zeiss 510 Meta confocal microscope with identical laser and detection settings.
Disease severity was graded histologically by two blinded observers using defined disease activity parameters.
Sections were additionally evaluated by 3 blinded observers using histological-histochemical grading system proposed by Mankin et al. [ 52].
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