The generation and examination of these mutants both in vitro and in vivo can provide high-resolution analysis of biological processes and cell phenotypes and provide new insight into the evolution and molecular function of this important protein family.
Typically, deletion of these proteins leads to the abolishment of large PPI networks, highlighting the difficulty in examining the contributions of specific interactions/activities to complex biological processes and cell phenotypes.
Because of the multiplicity of risk factors, biological processes and cell types involved in atherosclerosis, therapeutic targeting to reduce growth or stabilize plaques is a challenging task.
DNase-Seq has emerged as a powerful genome-wide tool to identify and characterize chromatin transitions (DNase I hypersensitive sites or DHS) in regulatory regions across a range of biological processes and cell lines [ 1, 3- 5].
Since many biological processes and cell structures are susceptible to pressure changes [ 5], a counteracting action is expected to occur, most likely by controlling gene expression in a pressure-dependent way.
The annotation of mapped unigenes, including a thorough description of their molecular functions, biological processes and cell compartments involved, was determined based on Gene Ontology (GO) using the Blast2GO search tool [ 56].
