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The GluN1 cytoplasmic domain (Fig. 2C) also binds to α-actinin and the binding region is located in the α-actinin rod domain.

Cryoelectron microscopy of RRV complexed with the Fab fragment of MAb T10C9, which binds in the vicinity of the E2-216 revealed revealed that the binding region is located at the outermost tip of the E2 glycoprotein [55].

This is in line with data showing that the functional CSA binding region is located N-terminally allowing for non-blocking antibodies to react with the C-terminal located domains (Dahlbäck et al submitted).

However, we cannot exclude that the Hoxb4 3'UTR binding region is located in a sequence bridging R1 to R2 or R2 to R3.

This conserved binding region is located within the CoZi domain.

The N-acetylglutamate synthase domain, as well as an acetyl-CoA binding region, is located at the C-terminus of ArgA.

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Furthermore, a significant fraction of binding regions is located at a significant distance from the nearest TSS and associated promoter and/or is even intragenic, an observation made for bacterial [ 46– 49] and for archaeal TFs as well [ 16, 50].

The first of these binding regions is located at the polar headgroup of thiazolidinediones (TZDs), interacting with the H12 helix of the LBD, and the second is between the distal end of the TZDs and the entrance of the ligand binding site.

Similar to HA, the receptor-binding region is located at the top of the head domain, which consists only of HEF1 residues.

A presumptive ATP-binding region is located at the N-terminal pole of the TK domain and contains a conserved lysine (K606, Figure 7, black background) responsible for binding ATP.

Interestingly, only a small proportion of those genes are involved in N assimilation and metabolism, and 65% of the binding regions were located intragenically.

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