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Consistent with the report by Fatemi et al. [14], Suetake et al. [15] reported that the DNA binding activity is located in the N-terminal amino acids 119 197.
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A region of high regulatory activity is located around 5 kb downstream of this variant, including binding sites for transcription factors, including GATA1, GATA2, GATA3, and NR2F2.
Most polymorphisms causing substrate-specific loss of OCT1 activity are located in the transmembrane helices that are known to be involved in the formation of the binding cleft (Fig. 8) [ 50].
The specific reaction of maleimide with the single thiol group ensures that full binding activity is retained, because the thiol group is located in the concave side of the protein whereas the four PS binding domains of anxA5 are in the convex side.
We previously reported that the Fn-binding activity of BBK32 is located to an intrinsically disordered segment of the protein corresponding to residues 21 205 [19] and that a motif corresponding to residues 147 205 binds to the 1F1 5F1 N-terminal segment of Fn by the tandem β-zipper mechanism [19], [20].
We assumed that the gp120 serum binding activity was due to glycan-specific antibodies.
NF-κB binding activity was also increased markedly.
Pleasingly, simultaneous binding activity was confirmed.
DNA binding activity was assessed by EMSA.
The RGD binding site is located at FNIII10 and is recognized by integrins α5β1, αVβ1 and αVβ3 (red and blue).
This conserved binding region is located within the CoZi domain.
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