Three independent analyses unequivocally confirm that the MBD binds to DNA as a dimer; stoichiometric titration, analytical sedimentation equilibrium, and binding isotherm analysis.
Analysis of the binding isotherm data suggests there is electrostatic repulsion between protein molecules when PnA binds GM1, while CTB GM1 demonstrates positive ligand ligand cooperativity.
Bottom panel: the binding isotherm for PXL2LRR-CLE42 interaction.
The XRD spectra were obtained after the chloride binding isotherm experiments were complete.
Furthermore, a chloride binding isotherm is offered that is compared with Freundlich and Langmuir models.
HVA MIP prepared using this formulation gives rise to a binding isotherm that is accurately modelled by the Freundlich isotherm.
Moreover, the degree of MB ion complexation with the PSS polyelectrolyte was investigated using the Langmuir-type binding isotherm.
A binding isotherm for NR at GC-DNA electrode was obtained from coulometric titrations and gave an affinity constant equal to 2.76 × 104 L mol−1.
This model accounts for the non-linear chloride binding isotherm and the non-equilibrium conditions between the free- and bound-chloride concentrations in concrete.
Therefore it is very important to define and model properly the chloride binding isotherm and its influence on free chloride transport.
The data were analyzed and the binding isotherm was fitted to a single-site model in the ORIGIN 7.0 software (GE Life Sciences).
