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With respect to community structure, the shifting baseline does not have an inherently negative connotation; it simply means that similarity of communities tends to decrease between two samples as the time between samples increases (i.e., progressive change) [9].
We quantified expression changes between two samples as the mean logarithm (log10) expression ratio (MLR) compared with a reference pool RNA sample.
Methods: We characterize differential transcription between two samples as the difference in the relative abundance of the transcript isoforms present in the samples.
Furthermore, as is often the need in biomedical research, this approach will let us study in finer detail the differences between two samples as captured by their genetic makeup.
Differential gene expression studies typically use the fold change statistic (the ratio of mRNA quantities between two samples) as input, and have been used to discover genes involved in adaptive stress responses which have not been previously characterized (i.e., "novel genes") [ 1].
Similar(55)
There is good discrimination between the two samples, as well as many commonly expressed genes.
A core region with a certain number (default 5) of CpG dinucleotides is used as the seed to call differentially methylated regions between two samples, such as 'cancer' and 'normal' samples.
A comparison between two samples named as A (without AlN buffer layer) and B (with an AlN buffer layer about 60 nm) was performed to assess the function of the buffer layer.
Recently, microarray has become the basic tool to search gene expression differences between two samples such as normal cells and cancer cells.
However, the relative expression of a gene between two samples should be directly comparable, as long as the normalization factor is comparable.
To confirm differentially expressed proteins between the treatment (B-CK) and control (A-CK), in the present study, only proteins that were identified with more than 1.5-fold changes and p value ≤0.05 between two samples were considered as differentially expressed proteins.
Related(20)
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