Sentence examples for before precipitation from inspiring English sources

Promoter regions cloned from genomic DNA prepared from VCaP cells using a high salt extraction buffer (10 m M Tris, pH 7.5, 400 m M NaCl, 100 m M EDTA, 0.6% SDS), protein precipitated with 6  M NaCl before precipitation of DNA using one sample volume of 100% ethanol.

The concentrations of aqueous components increase monotonically before precipitation occurs (Figure 5).

Control of particle size by regulation of salt concentration looks preferably since ZHP obtained by this manner accelerates sorption despite initial ZrOCl2 content in the resin before precipitation.

Then, a sudden drop in the air temperature occurred in the late afternoon, starting about 10 min before precipitation was observed at the observation site.

It was observed that the simultaneous evolution of particle growth and agglomeration is controlled if HPMC LV 15 and PF 127 are present before precipitation while only agglomeration is suppressed if added after precipitation.

Increasing in concentration of salt, which is used for impregnation of the ion-exchanger before precipitation (increase of ( {left[{overline{mathrm{Cat}}}^right]}_{mathrm{ad}} )), and decreasing in acid (precipitator) concentration also cause precipitation of smaller particles inside the polymer.

The products selectivity variation trend indicated that the selectivity to gaseous and light hydrocarbons was increased, whereas that to heavy hydrocarbons and olefin was suppressed when the Zr species and SiO2 were precipitated before the precipitation of Fe3+ cation.

Input DNA before immune precipitation (IN) and immune-precipitated DNA using an anti-HA antibody (IP) was analyzed by qPCR employing gene-specific primer pairs (p) indicated in the IGV graph.

Pre-treatment with polyvinylpyrrolidone (PVP) or polyvinylpolypyrrolidone (PVPP) before DNA precipitation to bind the phenolic components before DNA precipitation has been suggested as a helpful step in the purification process [ 20, 21, 42] but to our experience the recovered DNA may not be sufficiently pure for enzymatic reactions.

For DNA agarose gel analyses, after the polymerization assays using incubations with the amphibian extracts, some samples were treated during 1 hr at 55°C with Proteinase K (10 U/assay), then phenol/chloroform and chloroform (V/V) treated before ethanol precipitation and totally loaded in an 1% agarose native gel before electrophoresis in TEA 1X buffer as described by Maniatis and Sambrook [28].

An explanation for this relationship is proposed here based on the temperature gradient measured before the precipitation events.