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Justyna Jupowicz-Kozak quote

Justyna Jupowicz-Kozak

CEO of Professional Science Editing for Scientists @ prosciediting.com

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before further washing

Grammar usage guide and real-world examples

USAGE SUMMARY

The phrase "before further washing" is correct and usable in written English.
It can be used to indicate a step that should be taken prior to additional washing, often in contexts related to cleaning or processing items. Example: "Make sure to rinse the vegetables before further washing to remove any dirt."

✓ Grammatically correct

Science

Wiki

Human-verified examples from authoritative sources

Exact Expressions

9 human-written examples

The peg lids were washed with distilled water, and the attached cell material was then stained with 0.1% crystal violet solution (5% methanol, 5% isopropanol) before further washing to remove excess dye.

Cells were then washed twice in PBS and fixed again with 3% formaldehyde (5 min) before further washing.

Cells were incubated at 4°C for 20 minutes before further washing in FACS buffer.

Science

Plosone

Species-specific fluorochrome-conjugated secondary antibodies (Invitrogen) were then applied for 1 2 hrs, before further washing and mounting in Vectashield mounting medium (Vector Laboratories , Inc. containing 100 ng/ml 4,6-diamidino-2-phenylindole (DAPI).

Science

Plosone

Membranes were washed (3×5 min) with TBS-T and incubated for 1 h at room temperature with HRP-conjugated anti-rabbit secondary antibody (New England Biolabs, UK), before further washing (3×5 min) with TBS-T and incubation for 5 min with ECL reagents (enhanced chemiluminescence kit, Immunstar; Bio-Rad).

Science

Plosone

Cells on day 10 were stimulated with peptides, or were unstimulated, for 20 hours, the last 18 hours of which were in the presence of 0.1 µg/ml Brefeldin A. Cells were washed, stained with surface antibodies (CD3, CD4, CD8) for 20 minutes before further washing and addition of Perm/Fix for 20 mins.

Science

Plosone
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Human-verified similar examples from authoritative sources

Similar Expressions

51 human-written examples

A further washing step was then performed before incubation with the second primary antibody.

A further washing step was then performed before incubation with the Ki67 antibody.

After further washing steps cells were resuspended in FACS-buffer before analyses.

Science

Plosone

MAC-T cells were further washed as before and transwells were removed from the supports and transferred to a new sterile 12 well plate.

Membranes were further washed five times with TBST before ECL exposure.

Science

eLife
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Expert writing Tips

Best practice

When using "before further washing", ensure that the initial washing step is clearly defined to avoid ambiguity. Specify the washing agent or method for clarity.

Common error

Avoid using "before further washing" without specifying what the initial wash entails. For example, instead of "the cells were processed before further washing", write "the cells were processed after an initial wash with PBS before further washing with distilled water".

Antonio Rotolo, PhD - Digital Humanist | Computational Linguist | CEO @Ludwig.guru

Antonio Rotolo, PhD

Digital Humanist | Computational Linguist | CEO @Ludwig.guru

Source & Trust

76%

Authority and reliability

4.4/5

Expert rating

Real-world application tested

Linguistic Context

The phrase "before further washing" functions as a prepositional phrase indicating the timing of an action in relation to a washing process. It specifies that something occurs prior to an additional or subsequent wash, building upon an initial cleaning step. As Ludwig AI indicates, this is a correct and usable construction.

Expression frequency: Uncommon

Frequent in

Science

80%

Wiki

20%

News & Media

0%

Less common in

Formal & Business

0%

Encyclopedias

0%

Reference

0%

Ludwig's WRAP-UP

In summary, the phrase "before further washing" is a grammatically sound and commonly used prepositional phrase, as per Ludwig AI analysis. It's used to specify the timing of an action in relation to a cleaning or washing process, particularly in scientific contexts. While the phrase is correct, it's important to clearly define the initial washing step to prevent ambiguity. Some alternatives include "prior to additional washing" and "preceding subsequent washing". The frequency of the expression can be classified as uncommon.

FAQs

How is "before further washing" used in a sentence?

The phrase "before further washing" indicates an action or treatment that occurs prior to an additional washing or rinsing step. For example, "The samples were incubated in the solution "before further washing" with distilled water".

What can I say instead of "before further washing"?

You can use alternatives like "prior to additional washing", "preceding subsequent washing", or "before the next washing cycle depending on the context.

Is it correct to say "prior to further washing" instead of "before further washing"?

Yes, "prior to further washing" is a grammatically correct and more formal alternative to "before further washing". Both phrases indicate that something should happen "before further washing" takes place.

What is the difference between "before washing" and "before further washing"?

"Before washing" generally refers to the initial cleaning step, whereas ""before further washing"" implies there has already been an initial wash or cleaning process and more washing is required.

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Source & Trust

76%

Authority and reliability

4.4/5

Expert rating

Real-world application tested

Most frequent sentences: